Vnitr Lek 2025, 71(4):E14-E21 | DOI: 10.36290/vnl.2025.049
Antibiograms in clinical practice
- Ústav mikrobiologie LF UP a FN Olomouc
The antibiogram is a fundamental diagnostic instrument for the appropriate selection and optimization of antimicrobial therapy in clinical practice. It is an essential component of antibiotic stewardship, which, through collaboration between clinical microbiologists and treating physicians, enables optimal treatment outcomes in bacterial infections, minimization of adverse effects, prevention of antibiotic resistance development, and cost-effective treatment. This review article summarizes various antibiogram methodologies and their determination procedures, with particular emphasis on the interpretation and practical implementation of antibiograms in routine clinical practice.
Keywords: bacteria, antibiotics, susceptibility, resistance, interpretation.
Accepted: June 3, 2025; Published: June 19, 2025 Show citation
References
- Luna CM, Vujacich P, Niederman MS, et al. Impact of BAL data on the therapy and outcome of ventilator-associated pneumonia. Chest.1997;111:676-685.
Go to original source...
Go to PubMed...
- Tumbarello M, Sanguinetti M, Montuori E, et al. Predictors of mortality in patients with bloodstream infections caused by extended-spectrum-ß-lactamase-producing Enterobacteriaceae: importance of inadequate initial antimicrobial treatment. Antimicrob Agents Chemother.2007;51:1987-1994.
Go to original source...
- Rello J, Torres A, Ricart M, et al. Ventilator-associated pneumonia by Staphylococcus aureus. Comparison of methicillin-resistant and methicillin-sensitive episodes. Am J Respir Crit Care Med.1994;150:1545-1549.
Go to original source...
Go to PubMed...
- Kolář M. Bacterial infections, antimicrobial resistance and antibiotic therapy. Life.2022;12:468.
Go to original source...
Go to PubMed...
- Laxminarayan R, Duse A, Wattal C, et al. Antibiotic resistance-the need for global solutions. Lancet Infect Dis.2013;13:1057-1098.
Go to original source...
Go to PubMed...
- Kolář M. Stoupající odolnost bakterií k antibiotikům - výzva pro farmakoterapii 21. století. Acta Med.2012;6:9-12.
- Luyt CE, Bréchot N, Trouillet JL, Chastre J. Antibiotic stewardship in the intensive care unit. Crit Care.2014;18:480.
Go to original source...
Go to PubMed...
- Dyar OJ, Huttner B, Schouten J, et al. What is antimicrobial stewardship? Clin Microbiol Infect.2017;23:793-798.
Go to original source...
Go to PubMed...
- Srinivasan A. Antibiotic stewardship: Why we must, how we can. Cleve Clin J Med.2017;84:673-679.
Go to original source...
Go to PubMed...
- Kolar M, Htoutou Sedlakova M, Urbanek K, et al. Implementation of antibiotic stewardship in a university hospital setting. Antibiotics.2021;10:93.
Go to original source...
Go to PubMed...
- Kolář M. Klinická mikrobiologie a její role v současné medicíně. Vnitr Lek.2023;69:E11-E14.
Go to original source...
Go to PubMed...
- Luyt CE, Bréchot N, Trouillet JL, Chastre J. Antibiotic stewardship in the intensive care unit. Crit Care.2014;18:480.
Go to original source...
Go to PubMed...
- European Committee on Antimicrobial Susceptibility Testing. Available from: http://www.eucast.org.
- Gajic I, Kabic J, Kekic D, et al. Antimicrobial susceptibility testing: a comprehensive review of currently used methods. Antibiotics.2022;11:42.
Go to original source...
Go to PubMed...
- Calderaro A, Chezzi C. MALDI-TOF MS: A reliable tool in the real life of the clinical microbiology laboratory. Microorganisms.2024;12:322.
Go to original source...
Go to PubMed...
- Htoutou Sedláková M, Bogdanová K, Kolář M. Novinky v oblasti bakteriologického vyšetření krve. Vnitr Lek.2024;70:174-179.
Go to original source...
- Jacoby GA. AmpC beta-lactamases. Clin Microbiol Rev.2009;22:161-82.
Go to original source...
Go to PubMed...
- Tamma PD, Aitken SL, Bonomo RA, et al. Infectious Diseases Society of America 2023 guidance on the treatment of antimicrobial resistant Gram-negative infections. Clin Infect Dis.2023;18:ciad428.
Go to original source...
Go to PubMed...
- Magiorakos AP, Srinivasan A, Carey RB, et al. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect.2012;18:268-281.
Go to original source...
Go to PubMed...
- Kadri SS, Adjemian J, Lai YL, et al. Difficult-to-treat resistance in Gram-negative bacteremia at 173 US hospitals: retrospective cohort analysis of prevalence, predictors, and outcome of resistance to all first-line agents. Clin Infect Dis.2018;67:1803-1814.
Go to original source...
Go to PubMed...