Vnitr Lek 1990, 36(9):873-878

[The HLA crossmatching test before thrombocyte transfusion. Comparison of 3 methods].

A Májský, J Prazák: Ustav hematologie a krevní transfúze, Praha.

Klíčová slova: Blood Transfusion; Coombs Test; Cytotoxicity Tests, Immunologic; HLA Antigens, analysis, ; Histocompatibility Testing, methods, ; Humans; Platelet Transfusion

Weak accessory HLA antibodies in HLA sera were assessed by three test--the microlymphocytotoxic, antiglobulin cytotoxic and fluorescent cytotoxic test. Most frequently they were detected by the antiglobulin cytotoxic test (69.64%), followed by the fluorescent cytotoxic test (55.73%) and least frequently by the microlymphocytotoxic test (45.77%). From the results ensues that the antiglobulin cytotoxic test is a suitable method of crossmatch before thrombocyte transfusions to patients who developed reactions after transfusions or where therapy failed although the crossmatch was performed by the microlymphocytotoxic test between the donor's lymphocytes and the patient's serum and the test was negative.