Vnitr Lek 1991, 37(3):279-284

[HLA-DR antigen typing using the 2-color fluorescence test; the specificity and sensitivity of the method in comparison with the monocytotoxicity and B-lymphocytotoxicity tests].

A Májský

Ustav hematologie a krevní transfúze, Praha.

The author describes his own modification of a two-colour fluorescent technique for typing of HLA-DR antigens. Concurrent examination of HLA-DR antigens in 34 subjects by this method and the monocytotoxic or B-lymphocytotoxic test revealed a greater sensitivity of the fluorescent technique; there was however also a tendency of non-specific positivity of results. More reliable results were obtained when the fluorescent technique was performed on Hammat plates, as compared with examinations using Terasaki plates. To eliminate erroneous results, the author recommends to use for HLA-DR typing two methods concurrently.

Keywords: B-Lymphocytes, immunology, ; Cytotoxicity Tests, Immunologic; Fluorescent Antibody Technique; HLA-DR Antigens, analysis, ; Histocompatibility Testing; Humans; Monocytes, immunology, ; Sensitivity and Specificity

Published: March 1, 1991  Show citation