Vnitr Lek 2010, 56(Suppl 1):46-50

Use of platelet lysate for mesenchymal stem cell cultivation

E. Matějková^1,*, V. Foltánková², J. Michálek², B. Kubešová¹

¹ Tkáňová banka FN Brno, pracoviště Bohunice, přednostka prim. MUDr. Barbara Kubešová

² Univerzitní centrum buněčné imunoterapie, Babákův výzkumný institut Lékařské fakulty MU Brno, přednosta prof. MUDr. Roman Hájek, CSc.

Mesenchymal stem cells (MSCs) are a heterogeneous subset of stromal stem cells that can be isolated from many adult tissues. They can differentiate into cells of the mesodermal lineage, such as adipocytes, osteocytes and chondrocytes, as well as cells of other embryonic lineages. MSCs can interact with cells of both the innate and adaptive immune systems, leading to the modulation of several effector functions. After in vivo administration, MSCs induce peripheral tolerance and migrate to injured tissues, where they can inhibit the release of pro-inflammatory cytokines and promote the survival of damaged cells. This article recapitulates experimental data of MSC sources and mechanisms of MSC-isolation and cultivation according to GMP (good manufacture practice) standards.

Keywords: mesenchymal stem cells; platelet lysate

Received: April 2, 2010; Published: February 1, 2010  Show citation