Vnitr Lek 2006, 52(12):1194-1199

ZAP-70 in B-cell chronic lymphocytic leukemia: clinical significance and methods of detection

L. Smolej1,*, L. Saudková2, M. ©paček2, T. Kozák2
1 Oddělení klinické hematologie II. interní kliniky Lékařské fakulty UK a FN Hradec Králové, přednosta prof. MUDr. Jaroslav Malý, CSc.
2 Oddělení klinické hematologie 3. lékařské fakulty UK a FN Královské Vinohrady, Praha, přednosta doc. MUDr. Tomáą Kozák, Ph.D.

B-cell chronic lymphocytic leukemia (B-CLL) represents a heterogeneous disease with highly variable prognosis. Clinical staging systems (Rai, Binet) fail to accurately predict the prognosis of individual patients, especially in early stages. Modern prognostic markers, mainly the mutational status of the variable regions of immunoglobulin heavy chains (IgVH) and genetic aberrations, allow more accurate risk stratification. Assessment of the expression of intracellular protein tyrosine kinase ZAP-70 represents not only a potential surrogate marker for the technically difficult and routinely unavailable assessment of the IgVH mutational status, but might also be an independent prognostic factor. Study of ZAP-70 function in B-cells has broadened our knowledge on the pathogenesis of B-CLL. Routine ZAP-70 assessment has been hindered so far mainly by the lack of harmonization and standardization of the available methods of detection.

Keywords: B-cell chronic lymphocytic leukemia; ZAP-70; flow cytometry; immunohistochemistry; Western blotting; PCR; methylation status

Received: June 10, 2006; Accepted: September 13, 2006; Published: December 1, 2006  Show citation

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Smolej L, Saudková L, ©paček M, Kozák T. ZAP-70 in B-cell chronic lymphocytic leukemia: clinical significance and methods of detection. Vnitr Lek. 2006;52(12):1194-1199.
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    References

    1. Prausová D, Krejsek J, ®ák P et al. Význam imunofenotypového vyąetření v diagnostice B-lymfoproliferativních onemocnění. Vnitř Lék 2004; 50: 45-53. Go to PubMed...
    2. Papajík T, Jaroąová M, Plachý R et al. Chronická B-lymfocytární leukemie. Část I: Pohled na původ, biologii a genetické změny leukemických buněk. Trans Hemat dnes 2006; 12(2): 53-61.
    3. Hamblin TJ, Davis Z, Gardiner A et al. Unmutated Ig V(H) genes are associated with a more aggressive form of chronic lymphocytic leukemia. Blood 1999; 94: 1848-1854. Go to original source...
    4. Wiestner A, Rosenwald A, Barry TS et al. ZAP-70 expression identifies a chronic lymphocytic leukemia subtype with unmutated immunoglobulin genes, inferior clinical outcome, and distinct gene expression profile. Blood 2003; 101: 4944-4951. Go to original source... Go to PubMed...
    5. Rosenwald A, Alizadeh AA, Widhopf G et al. Relation of gene expression phenotype to immunoglobulin mutation genotype in B cell chronic lymphocytic leukemia. J Exp Med 2001; 194: 1639-1647. Go to original source... Go to PubMed...
    6. Chan AC, Iwashima M, Turck CW et al. ZAP-70: a 70 kd protein-tyrosine kinase that associates with the TCR zeta chain. Cell 1992; 71: 649-662 Go to original source... Go to PubMed...
    7. Nolz JC, Tschumper RC, Pittner BT et al. ZAP-70 is expressed by a subset of normal human B-lymphocytes displaying an activated phenotype. Leukemia 2005; 19: 1018-1024. Go to original source... Go to PubMed...
    8. Orchard J, Ibbotson R, Best G et al. ZAP-70 in B-cell malignancies. Leuk Lymphoma 2005; 46: 1689-1698. Go to original source... Go to PubMed...
    9. Rassenti L, Huynh L, Toy TL et al. ZAP-70 compared with immunoglobulin heavy-chain gene mutation status as a predictor of disease progression in chronic lymphocytic leukemia. N Engl J Med 2004; 351: 893-901. Go to original source... Go to PubMed...
    10. Orchard J, Ibbotson R, Davis Z et al. ZAP-70 expression and prognosis in chronic lymphocytic leukemia. Lancet 2004; 363: 105-111. Go to original source... Go to PubMed...
    11. Crespo M, Bosch F, Villamor N et al. ZAP-70 expression as a surrogate for immunoglobulin-variable-region mutations in chronic lymphocytic leukemia. N Engl J Med 2003; 348: 1764-1775. Go to original source... Go to PubMed...
    12. Dürig J, Nückel H, Cremer M et al. ZAP-70 expression is a prognostic factor in chronic lymphocytic leukemia. Leukemia 2003; 17: 2426-2434. Go to original source... Go to PubMed...
    13. Orchard J, Best OG, Ibbotson RE et al. ZAP-70 by flow cytometry: Comparison of methodologies, and with IgVH mutational nad ZAP-70 methylation status. (ASH Annual Meeting Abstracts) 2005; 106: 1181. Go to original source...
    14. Gibbs G, Bromidge T, Howe D et al. Comparison of flow cytometric methods for the measurement of ZAP-70 expression in a routine diagnostic laboratory. Clin Lab Haem 2005; 27: 258-266. Go to original source... Go to PubMed...
    15. Jorgensen J, Jones D, Luthra R et al. Flow cytometric detection of ZAP-70 in chronic lymphocytis leukemia: addition of a novel second monoclonal antibody improves correlation with IgVH mutation status. Blood (ASH Annual Meeting Abstracts) 2005; 106: 3277. Go to original source...
    16. Letestu R, Baran-Marszak F, Lefebvre V et al. Evaluation of ZAP-70 expression using real-time quantitative RT PCR (RQ-PCR) in chronic lymphocytic leukemia (CLL), a reference method for flow cytometry (FCM) harmonization? Blood (ASH Annual Meeting Abstracts) 2005; 106: 1195. Go to original source...
    17. Kay S, Herishanu Y, Pick M et al. Quantitative flow cytometry of ZAP-70 levels in chronic lymphocytic leukemia using molecules of equivalent soluble fluorochrome. Cytometry B Clin Cytom 2006; 70: 218-226. Go to original source... Go to PubMed...
    18. Sup SJ, Domiati-Saad R, Kelley TW et al. ZAP-70 expression in B-cell hematologic malignancy is not limited to CLL/SLL. Am J Clin Pathol 2004; 122: 582-587. Go to original source...
    19. Admirand JH, Rassidakis GZ, Abruzzo LV et al. Immunohistochemical detection of ZAP-70 in 341 cases of non-Hodgkin and Hodgkin lymphoma. Mod Pathol 2004; 17: 954-961. Go to original source... Go to PubMed...
    20. Carreras J, Villamor N, Colomo L et al. Immunohistochemical analysis of ZAP-70 expression in B-cell lymphoid neoplasms. J Pathol 2005; 205: 507-513. Go to original source... Go to PubMed...
    21. Wang J, Young L, Win W et al. Distribution and ZAP-70 expression of WHO lymphoma categories in Shanxi, China: a review of 447 cases using a tissue microarray technique. Appl Immunohistochem Mol Morphol 2005; 13: 323-332. Go to original source... Go to PubMed...
    22. Vener C, Gianelli U, Cortelezzi A et al. ZAP-70 immunoreactivity is a prognostic marker of disease progression in chronic lymphocytic leukemia. Leuk Lymphoma 2006; 47: 245-251. Go to original source... Go to PubMed...
    23. Kim SZ, Chow KU, Kukoc-Zivojnov N et al. Expression of ZAP-70 protein correlates with disease stage in chronic lymphocytic leukemia and is associated with, but not generally restricted to, non-mutated Ig VH status. Leuk Lymphoma 2004; 45: 2037-2045. Go to original source... Go to PubMed...
    24. Catherwood MA, Matthews C, Niblock R et al. ZAP-70 mRNA quantification in B-cell chronic lymphocytic leukaemia. Eur J Haematol. 2006; 76: 294-298. Go to original source... Go to PubMed...
    25. Laurenti L, Petlickovski A, Rumi C et al. Comparison of ZAP-70/Syk mRNA levels with immunoglobulin heavy-chain gene mutation status and disease progression in chronic lymphocytic leukemia. Haematologica 2005; 90: 1533-1540. Go to PubMed...
    26. Corcoran M, Parker A, Orchard J et al. ZAP-70 methylation status is associated with ZAP-70 expression status in chronic lymphocytic leukemia. Haematologica 2005; 90: 1078-1088. Go to PubMed...
    27. Binet J-L, Caligaris-Capio F, Catovsky D et al. Perspectives on the use of new diagnostic tools in the treatment of chronic lymphocytic leukemia. Blood 2006; 107: 859-861. Go to original source...
    28. Scielzo C, Camporeale A, Geuna M. ZAP-70 is expressed by normal and malignant human B-cell subsets of different maturational stage. Leukemia 2006; 20: 689-195. Go to original source... Go to PubMed...

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