Vnitr Lek 1999, 45(2):103-109

[Latent autoimmune (type I) diabetes mellitus in adults. Part. II. Association of HLA antigens, status of cellular immunity and occurrence of other autoimmune diseases].

E Martinka, I Shawkatová, J Straková, M Buc, M Mokán

I. interná klinika, JLF UK a MFN, Martin.

AIM: OF STUDY: To assess some immunological and immunogenetic aspects in patients with latent autoimmune (Type-1) diabetes mellitus (DM) of adults (LADA).
SUBJECTS: 24 patients with LADA, 11 patients with Type-2 DM and 20 healthy volunteers (Pilot study). PARAMETERS TESTED: HLA-DRB1* and HLA-DQB1* alleles, parameters of cellular immunity (CD4+, CD8+, CD3/HLA-DR+, CD8/HLA-DR+, CD45RA+[CD4], CD16+CD56), CD19+, IL-4, INF-gamma and organ specific (OSA) autoantibodies (against thyroid gland, gastric parietal cells, tubuli, basal membranes of glomerulus, AMA and ABBA).

RESULTS AND DISCUSSION: Type-1 DM HLA-DRB1* and HLA-DQB1* risk alleles have been found in a majority of patients with LADA. The most frequent were HLA-DRB1*0301 and DQB1*0201. Assessement of parameters of cellular immunity and cytokine profiles (IL-4 a INF-gamma) in peripheral blood did not reveal any contribution to a differentiation between Type-1 and Type-2 DM). We confirmed increased occurence of OSA in patients with LADA, what stress importance of routine screening for OSA in patients with LADA.

Keywords: Aged; Antigens, CD /analysis/; Autoantibodies /analysis/; Autoimmune Diseases /complications/; Diabetes Mellitus, Type 1 /complications/; Diabetes Mellitus, Type 2 /immunology/; Female; Gastric Mucosa /immunology/; HLA Antigens /analysis/; Humans; Interferon-gamma /metabolism/; Interleukin-4 /metabolism/; Lymphocyte Subsets; Male; Middle Aged; T-Lymphocytes, Helper-Inducer /immunology/; Thyroid Gland /immunology/

Published: February 1, 1999  Show citation