Vnitr Lek 1991, 37(9-10):789-794

[The effect of ethanol on increased expression of HLA-A,-B antigens on lymphocytes].

A Májský
Ustav hematologie a krevní transfúze, Praha.

Ethanol in a concentration of 0.001% to 2% increases the expressivity of HLA-A, B antigens on lymphocytes. In 26.13% of the examinations HLA antigens were proved only on lymphocytes processed with ethanol. The expressivity is maximally enhanced already by short-term incubation of lymphocytes with ethanol; if prolonged to 120 mins at 20 degrees C ethanol does not produce an unspecific positivity of results. There is no difference between processing of lymphocytes at 20 degrees C and 37 degrees C. 1% and 2% ethanol facilitates detection of HLA-A, B antigens after addition to serum and lymphocytes in the first stage of the microlymphocytotoxic test.

Keywords: Ethanol, pharmacology, ; HLA-A Antigens, analysis, ; HLA-B Antigens, analysis, ; Humans; Lymphocytes, immunology,

Published: September 1, 1991  Show citation

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Májský A. [The effect of ethanol on increased expression of HLA-A,-B antigens on lymphocytes]. Vnitr Lek. 1991;37(9-10):789-794.
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